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Lew Ayres
Dr. James Kildare
Lionel Barrymore
Dr. Leonard Gillespie
Laraine Day
Nurse Mary Lamont
Shepperd Strudwick
Dr. Gregory 'Greg' Lane
Samuel S. Hinds
Dr. Stephen Kildare
Emma Dunn
Mrs. Martha Kildare
Nat Pendleton
Joe Wayman, Ambulance Driver
Walter Kingsford
Dr. S.J. 'Walter' Carew, Hospital Administrator
Alma Kruger
Molly Byrd, Superintendant of Nurses
John Eldredge
Henry Adams, Amnesia Patient
Nell Craig
Nurse 'Nosey' Parker
Marie Blake
Sally, Hospital Switchboard Operator